recA Gene Sequence Analysis

recA gene is a good choice for species identification, and phylogenetic analysis of partial recA gene sequences has been successfully used for the identification of several strains.

Creative enzymes provide a strain identification method based on recA gene sequence analysis, offering the possibility of phylogenetic taxonomic markers for closely related species. We are committed to applying our science to provide our clients with fast and reliable strain identification services.

Introduction of recA Gene

recA is a small protein present in all bacteria due to its important function in homologous DNA recombination, DNA damage repair and induction of the SOS response. This panoply of functions implies multiple biochemical activities, including DNA binding (double and single stranded), pairing and exchange of homologous DNA, ATP hydrolysis, and coproteolitic cleavage of the LexA, λcI, and UmuD proteins. Based on the broad basal role of recA, its gene product has been proposed as a phylogenetic marker for distantly related species, especially among species with greater than 99% similarity.

Creative Enzymes obtained taxonomic and identification data of strains from the results of recA gene sequence analysis, focusing on Gram-negative and some Gram-positive bacteria.

Fig. 1 The four cellular functions of the RecA protein in Escherichia coli. (Patel, 2010)

Advantages of Applying recA Gene Sequence Analysis for Strain Identification

• Short recA gene sequences have high discriminatory power for indistinguishable species.
• The protein amino acid sequence of recA can be used to infer the phylogeny between distantly related taxa, thus avoiding the disadvantages of rRNA sequence analysis, e.g., overestimation of the relatedness of species with similar nucleotide frequencies, nonindependence of substitution patterns at different sites, and bias derived from different GC contents of the organisms.
• At the nucleotide level, recA can tolerate mutations that do not or only slightly alter its product. These mutations give us information about recent evolutionary history, which is too recent to be fixed in slowly diverging sequences like 16S rRNAs.

recA Gene Sequence Analysis Test Process

Creative Enzymes provides our clients with one-stop solutions for recA gene sequence analysis. All you need to do is provide us with the sample to be tested, and we promise to provide you with results containing comprehensive genus-specific information in the shortest possible time.

Fig. 2 recA gene sequence analysis test process of Creative Enzymes.

Advantages of Creative Enzymes

• Competitive price.
• Achieve the shortest delivery time.
• Provide scientific and reliable testing and analysis reports.
• Professional technical support and experienced project management.

Creative Enzymes is a professional and experienced probiotic supplier and service provider. We provide a method based on recA gene sequence analysis, which provides a good identification method for the analysis of similarly related strains. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.

Reference

1. Patel, M.; et al. A new model for SOS-induced mutagenesis: how recA protein activates DNA polymerase V. Critical reviews in biochemistry and molecular biology. 2010, 45(3): 171-184.