Molecular Biology Methods
Probiotics have positive health effects on the host when used in moderation. However, some probiotics may pose a potential health threat. Molecular biology methods are essential for the identification of bacterial strains used in marketed foods and pharmaceuticals, and the results are supportive for biochemical tests and phenotypic analysis for identification.
Creative Enzymes offers a variety of molecular biology methods to help our clients efficiently and accurately identify strain-related information in probiotic products.
Introduction of Molecular Biology Identification Methods
Molecular biology methods for strain identification are mainly based on polymerase chain reaction (PCR) and hybridization with nucleic acid molecular probes. PCR technology is primarily a technique for in vitro amplification of DNA fragments. PCR-based tests can provide a rapid, highly sensitive method for differentiating strains. Nucleic acid molecular probes are specific known nucleic acid fragments that can anneal and hybridize to complementary nucleic acid sequences and can therefore be used for the detection of specific gene sequences in the nucleic acid sample to be tested. Creative enzymes use different types of nucleic acid probes for strain identification depending on the purpose and class of the test.
Fig. 1 Molecular identification of strains.
Molecular Biology Identification Methods Provided by Creative Enzymes
Nucleic Acid Hybridization Technology
The use of nucleic acid hybridization tests to analyze the affinities between strains has become an important indicator of bacterial taxonomy. We offer nucleic acid hybridization identification services that requires only lysis of the strain to expose the intracellular DNA, allowing direct in situ hybridization to the colony using a probe.
Creative Enzymes provides our clients with professional and efficient nucleic acid molecular hybridization technology to identify strains in probiotic products. Our one-stop solution saves you time and effort and yields meaningful experimental results.
recA Gene Sequence Analysis
The recA protein encoded by the recA gene plays an important role in DNA recombination, DNA repair and SOS emergency response. Therefore, creative enzymes have designed efficient and sensitive molecular marker methods for interspecific evolutionary developmental affinity studies using recA gene fragments. Researches using this method in the genus Bifidobacterium have been successful.
rRNA Gene Sequence Analysis
Biomolecules such as rRNA in organisms are functionally stable and have both highly conserved and variable regions in their nucleic acid sequences, which are often used for the taxonomic identification of microorganisms. Creative enzymes amplify the 16S rRNA gene of the strain using primers common to bacteria. The sequences are determined and entered into a database for homology analysis to determine the classification of the strains.
Denaturing Gradient Gel Electrophoresis
Denaturing gradient gel electrophoresis is used to separate genomic fragments with subtle differences by gradient denaturing gel. Creative Enzymes uses denaturing gradient gel electrophoresis combined with sequencing analysis to help clients reveal the phylogenetic relationships of strains and thus better obtain taxonomic identification information for strains.
Creative Enzymes is a professional and experienced probiotic supplier and service provider. We offer a variety of molecular biology-based methods to help our clients identify strains efficiently and sensitively. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.
