Molecular Biology-Based Identification System
Automated molecular identification methods using the RiboPrinter system allow rapid generation of genetic fingerprints of strains for analysis against databases. Creative Enzymes' RiboPrinter system can efficiently and quickly identify strain information in probiotic products, which can be of great help to your probiotic development project.
Introduction of The RiboPrinter System
The RiboPrinter fully automated microbial genetic fingerprinting system is a powerful tool for rapid, accurate, high-resolution microbial strain level identification and molecular typing. Based on the unique genome of each microorganism, the RiboPrinter system integrates multiple modules such as DNA digestion, electrophoresis, blotting, molecular hybridization, imaging, standard strain database and automated bioinformatics analysis to automate and synchronize strain-level identification and molecular typing for standardized and efficient identification.
Principle of The RiboPrinter System
The basic principles of the RiboPrinter system are restriction endonuclease length polymorphism analysis and molecular blot hybridization (Southern Blot) analysis. Its work is divided into three main parts: whole genome digestion, DNA isolation and specific probe hybridization, fully automated data processing and bioinformatics analysis.
- Whole genome digestion
Enzymatic cleavage of the whole genome of microorganisms by restriction endonucleases produces a large number of DNA fragments. Due to the differences between strains, when the genomes of different strains are enzymatically cleaved, DNA fragments of the same or different lengths exist in the enzymatic cleavage products, and the differences in the target DNA fragments at the molecular level can be deciphered by specific probe hybridization.
- Electrophoresis and southern hybridization
The digested products are separated by electrophoresis of DNA fragments of varying sizes and then transferred to the membrane by Southern blotting. Identification and hybridization of specific DNA fragments on membranes by labeled, specific probes. The hybridization products can produce fingerprint bands with different positions and colors on the membrane after chemiluminescence.
- Bioinformatics analysis
The fingerprint was automatically analyzed by software, and the strains were identified and typed according to the band type and band number polymorphisms.
Workflow of The RiboPrinter System
- Sample preparation
A small amount of the sample to be analyzed is placed in a sample tube with buffer, stirred to suspend it in the buffer, and placed in a heater to inactivate it.
- On-machine testing
Place the sample in the RiboPrinter system and the system will automatically perform the following steps.
- DNA is extracted from bacterial cell lysates and fragmented with restriction endonucleases.
- Separation transfer is performed by gel electrophoresis to separate DNA fragments by their molecular weight and then transfer the DNA fragments to the membrane.
- After the assay is hybridized with a labeled DNA, a chemiluminescent reagent is introduced and the light emitted from the hybridized fragment is recorded by a digital camera and stored as image data.
- Data processing uses a dedicated mathematical algorithm model to provide a RiboPrinter pattern from each sample's image data for comparison with other patterns stored in the system to identify or characterize the sample.
- The print report system automatically prints the strain identification report.
Fig. 1 Testing prosess of the RiboPrinter System.
Advantages of The RiboPrinter System
- Easy to operate
Multiple strain analysis applications in one fully automated solution. The system is fully automated for tedious experimental steps, but that doesn't mean less functionality. On the contrary, the RiboPrinter system can present you with more valuable information.
- High resolution
Capable of extending the analysis to the whole genome level and performing high-resolution gene fingerprinting. It can provide basic information about strains and identify subtle differences between similar strains. The combined application of specific digestion and probe design also allows for higher resolution and a wider range of applications.
- Powerful and flexible data analysis and sharing platform
Powerful data analysis system that can quickly generate automated analysis reports. Standardized data can be easily shared and managed.
Creative Enzymes is a professional and experienced supplier and service provider of probiotics. Our scientific team has extensive experience and skills in microbial identification using the RiboPrinter system to help our clients better conduct relevant research. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.
