Denaturing Gradient Gel Electrophoresis

Denaturing gradient gel electrophoresis (DGGE), as a mature molecular biology technique, has been widely used for microbial analysis in food, medical and environmental fields.

Creative Enzymes provides our clients with a denaturing gradient gel electrophoresis-based strain identification method to help them obtain safe and edible probiotic products.

Introduction of Denaturing Gradient Gel Electrophoresis

Denaturing gradient gel electrophoresis (DGGE) was initially used to detect point mutations in DNA fragments and later developed into one of the important molecular biology methods used to research the structure of microbial communities. The principle of DGGE technique is to separate DNA fragments of the same length but different sequences based on the change in electrophoretic mobility caused by the different unstranding behavior of DNA fragments in different concentrations of denaturant. When DGGE technology is applied to the 16S rRNA gene, dissection of the microbial community at the phylogenetic level of its components can be achieved. PCR of regions of this gene using conserved primers can produce mixtures of amplicons that can be isolated by DGGE.

Fig. 1 Denaturing gradient gel electrophoresis.

Basis Using Denaturing Gradient Gel Electrophoresis for Probiotic Identification

Compared to complex ecosystems such as the human body (mouth, mucous membranes, intestines) and the environment (soil, oceans, rivers), probiotic products can be thought of as a simple microbial community. Based on this fact, qualitative analysis of any probiotic product can be performed using the DGGE technique.

Creative Enzymes offers DGGE technology that allows direct identification of amplicons at the species level, which can help clients to accurately analyze and validate microbial information in probiotic products in a short period of time.

Features of Denaturing Gradient Gel Electrophoresis

• The quality of probiotic products can be analyzed and controlled in a fast and reliable way.
• Avoids the disadvantage that most probiotic analyses need to involve the use of specific isolation media and the identification of a limited number of isolates.
• A method is provided to analyze microorganisms in a community without the need for a culture method.

Strain Identification Process of Creative Enzymes

Creative Enzymes provides our clients with one-stop solutions for the analysis and identification of probiotics. We offer a denaturing gradient gel electrophoresis method that is independent of strain culture and allows for the extraction of total bacterial DNA directly from the product, PCR amplification of the V3 region of 16S ribosomal DNA, and amplicon isolation on denaturing gradient gels.

Fig. 2 Strain identification by denaturing gradient gel electrophoresis (DGGE).

All you need to do is provide us with the sample to be tested and we promise to provide you with results containing comprehensive species-specific information in the shortest possible time.

Advantages of Creative Enzymes

• Competitive price.
• Achieve the shortest delivery time.
• Provide scientific and reliable experimental analysis reports.
• Professional technical support and experienced project management.

Creative Enzymes is a professional and experienced probiotic supplier and service provider. Our scientific team offers clients a method to analyze strain information in probiotic products based on denaturing gradient gels electrophoresis. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.