Nucleic Acid Hybridization Technology
Nucleic acid hybridization techniques are valuable for the taxonomic identification of strains with small differences in phenotypic traits that are difficult to identify, and are important for solving taxonomic problems above the species level and for the identification of new species.
Creative Enzymes offers nucleic acid hybridization technology to provide our clients with a more reliable method of taxonomic identification by directly comparing genomic differences between different microorganisms. All you need to do is provide the sample to be tested and we will provide you with results containing comprehensive genus-specific information within the agreed period.
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DNA-DNA Hybridization
The DNA-DNA hybridization method we offer is based on the principle that DNA from different sources is unstranded by in vitro heating, and under suitable conditions, complementary bases are recombined to form double-stranded DNA.
The percentage of heterozygosity is detected according to the ability to generate double chains. If the base order of two single-stranded DNAs are identical, they can produce a complete double-stranded DNA, i.e., the heterozygosity rate is 100%. If the base sequences of two single-stranded DNAs are only partially identical, they can produce a "double strand" containing only partial single strands, and the heterozygosity rate is less than 100%. Therefore, the higher the heterozygosity rate, the closer the affinity between the two strains. This method provides an accurate method for the taxonomic identification of strains.
Fig. 1 Schematic diagram of strain identification using DNA-DNA hybridization.
DNA-rRNA hybridization
Although DNA-DNA hybridization technology solves some difficult problems that cannot be solved based on phenotypic characteristics. However, when the DNA pairing base of the two strains is less than 20%, DNA-DNA molecule hybridization often fails to form a double strand, limiting the use of this method. Fortunately, when the DNA-DNA hybridization rate between two strains is low or not possible, a high hybridization rate may still occur with DNA-rRNA hybridization. Thus, it can be used to further compare the relationships between more distantly related strains for the classification of taxonomic units at the genus and above genus level.
The principles and methods of DNA-rRNA hybridization and DNA-DNA hybridization are basically the same, with the differences being:
- In DNA-rRNA molecular hybridization, the isotope labeling site is at the rRNA.
- The results of DNA-rRNA molecular hybridization are expressed as Tm values; higher Tm values indicate closer affinity.
Testing Process of Creative Enzymes
Creative Enzymes utilizes our advanced technology platform to provide professional, comprehensive one-stop strain identification services to our clients. Our testing process is described below. If you want to get more information, you can consult us at any time.
Fig. 2 Strain identification process.
Advantages of Creative Enzymes
- Competitive price.
- Achieve the shortest delivery time.
- Provide scientific and reliable testing and analysis reports.
- Professional technical support and experienced project management.
Creative Enzymes is a professional and experienced probiotic supplier and service provider. We offer nucleic acid hybridization-based identification methods to help our clients obtain strain-related information efficiently and accurately. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.
