In Vitro Safety Assessment of Probiotics
In vitro assessment is the research that addresses the essential properties of probiotic strains. Creative Enzymes is a specialized provider of probiotic safety assessment services, providing our clients with multiple tests to analyze the safety and efficacy of their products. Creative Enzymes is committed to building close relationships with our clients to provide comprehensive and reliable solutions for their high-end probiotic product development projects to accelerate product development and launch.
The Significance of In Vitro Safety Assessment of Probiotics
Probiotics serve as a food, feed or medicine containing live microorganisms that, when ingested, confer beneficial physiological effects on the host animal through microbial action. For probiotic strains used in humans and animals, it is essential to study their safety in vitro through multiple perspectives.
Factors to Consider in The In Vitro Safety Assessment of Probiotics
Factors that must be considered when assessing the safety of probiotics are pathogenicity, infectivity and virulence factors, including toxicity, metabolic activity and intrinsic properties of the microorganism. However, it is worth noting that the classical evaluation approaches traditionally used for safety assessment of pathogenic bacteria is not necessarily applicable to probiotic strains. Pathogenicity of pathogenic bacteria is usually exhibited by a combination of multiple properties of the strain.
As experienced experts in probiotic research, Creative Enzymes' expertise and attention to detail allow us to provide you with quality services to help you address the challenges of evaluating the in vitro safety of your probiotic products.
In Vitro Safety Assessment Services of Probiotics at Creative Enzymes
Creative Enzymes has extensive expertise and practical experience in evaluating the safety of probiotics. We offer a variety of in vitro assessment methods that combine multiple factors to help our clients analyze whether their probiotic strains meet the required safety standards. We provide in vitro assessment methods for probiotics focusing on various aspects such as pathogenicity, metabolite toxicity, and adhesion, but not limited to the following.
Fig. 1 In vitro safety assessment of probiotics.
Probiotics Antibiotic Resistance Test
In clinical, probiotic strains that are resistant to a particular antibiotic are usually associated with infections. Creative Enzymes uses a variety of tests to systematically screen and evaluate antibiotic resistance gene susceptibility for microorganisms that will be used as probiotics.
Detection of Pathogenicity and Production of Toxic Metabolites of Probiotics
The absence of pathogenicity and the non-production of toxic substances through metabolism are necessary conditions for the safety of probiotics. Creative Enzymes uses our science to evaluate the potential for probiotics to contain potentially pathogenic genes as well as toxic metabolites to provide safe and reliable probiotic products to the market.
Probiotics Adhesion Tests to Host Tissues
Adherence is important for probiotics to function, while for pathogenic bacteria it is a negative effect that affects translocation and infection. Creative enzymes conduct research on the adhesion and colonization of probiotics to help our clients screen probiotic products.
Platelet Agglutination Test of Probiotics
Platelet aggregation activity is considered a necessary test for safety assessment due to its association with factors such as pathogenic bacteria that promote infection. Creative enzymes analyze the safety of probiotics by testing their platelet agglutination activity.
Hemolysis Test of Probiotics
Hemolytic effect is one of the pathogenic mechanisms of pathogenic bacteria. Creative Enzymes utilizes advanced technology to evaluate and confirm that your probiotic product is safe and reliable.
Immunomodulatory Risk Assessment of Probiotics
Some probiotics exert their probiotic effects through immune stimulation. However, probiotics that cause harmful inflammation or autoimmunity in an immunocompromised host can cause hypersensitivity reactions in hypersensitive individuals. Creative Enzymes incorporates the potential risk of immune effects of probiotics into the safety assessment to help clients gain a deeper and more comprehensive understanding of the quality and safety of probiotics.
Bile Salt Degradation Activity of Probiotics
The degradation of bile salts by probiotics may lead to malabsorption and may cause and promote the development and progression of colon cancer due to the early dissociation of bile salts to produce bile acids. Creative Enzymes utilizes our extensive testing platform to test the bile salt degradation ability of probiotics to facilitate the evaluation of their safety.
Mucin Degradation Test of Probiotics
Certain microorganisms in the gut produce enzymes that break down mucosal proteins, causing cell damage and increasing the risk of infection. Creative Enzymes utilizes our advanced platform to measure enzyme activity in probiotics associated with the degradation of intestinal mucus glycoproteins to provide insight into their in vitro safety.
Creative Enzymes is a professional and experienced probiotic supplier and service provider. Creative Enzymes provides comprehensive in vitro safety assessment solutions from all aspects to accelerate our clients' potential probiotic product screening and evaluation programs. To get more information, please contact us and we will be happy to serve your research demands.
