Preservation of Probiotic Strains

The preservation method and shelf life of probiotic strains vary depending on the strain.

Creative Enzymes, as an experienced probiotic-related service provider, selects appropriate preservation methods for customers according to the growth characteristics of different strains of bacteria.

Importance of Strain Preservation

• Excellent strains of bacteria are important biological resources for biological research and development of new probiotic products.
• When a promising strain is found or selected, it is important to keep its good traits unchanged or change as little and as slowly as possible in order not to reduce its production performance.

The Principle of Culture Preservation

According to the physiological and biochemical characteristics of different strains, artificially create conditions to make the metabolism of microorganisms in a semi-permanent dormant state of inactivity, inhibition of growth and reproduction. These artificially caused environments are mainly low temperature, dryness, and lack of oxygen, which can make the strain rarely mutate to achieve the purpose of maintaining pure breeds.

The Probiotic Preservation Services We Offered

Subculturing Preservation Method

Subculturing is the most basic microbial preservation method, which requires regular transfer and culture of strains before preservation. For example, the preservation of commonly produced strains such as yogurt. This method is very friendly to strains that die quickly when exposed to treatments such as freezing or drying.

Liquid Paraffin Covered Preservation Method

It is a disguised method of subculturing, which can extend the preservation time appropriately. It is covered with sterilized liquid paraffin on top of slant cultures and puncture cultures, which can prevent the death of the strain due to the evaporation of water from the medium on the one hand, and prevent the entry of oxygen to weaken the metabolic effect on the other.

Carrier Preservation Method

Preservation methods in which microorganisms are adsorbed on suitable carriers, such as soil, sand, silica gel, or filter paper, and then dried, such as sand preservation and filter paper preservation, are quite widely used.

Freeze Vacuum Drying Method

This preservation method takes advantage of the principle that microorganisms are dormant when they are at low temperatures, isolated from air and dry conditions. The preserved strains are dispersed in a protective agent and kept under vacuum conditions of low temperature freezing, and the ice sublimates and dries the strains.

Cryopreservation Method

Suitable for microorganisms that are resistant to freezing. Commonly used cryopreservation methods include:

• Cryogenic refrigerator storage method (-20°C, -50°C or -85°C).
• Dry ice preservation method (around -70°C).
• Liquid nitrogen preservation method (-196°C).

Creative Enzymes is a professional and experienced probiotic supplier and service provider. We are committed to providing suitable, customized strain preservation solutions for our clients' excellent probiotic strains. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.