Production and Identification of Prebiotics
Prebiotics can be incorporated into many food products such as beverages, health and sports drinks, infant formulas, cereals, breads, savory products, etc., and have received widespread commercial attention. Creative Enzymes uses our science to help clients rationally develop prebiotics to achieve specific functional properties and health outcomes.
Fig. 1 Sources and production of major prebiotics, including fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS). (Davani-Davari, 2019)
Types of Prebiotics
There are many types of prebiotics, most of them are a subset of the carbohydrate group, mainly oligosaccharide carbohydrates, but also include other classes of compounds. Creative Enzymes can provide any prebiotic development and research services to our clients, including but not limited to the following.
| Different Types of Prebiotics | |
|---|---|
| Lactose-derived prebiotics | Galacto-oligosaccharides, Lactosucrose, Lactulose |
| Sucrose-derived prebiotics | Fructo-oligosaccharides, Isomaltulose (palatinose) |
| Starch-derived oligosaccharides | Malto-oligosaccharides, Isomaltose oligosaccharide, Isomaltotriose |
| Xylan-derived prebiotics | Xylo-oligosaccharides, Arabinoxylo-oligosaccharides |
Our Services
- Production of Prebiotics
Prebiotics can be produced in different ways, depending on the availability of the source. Creative Enzymes helps our clients obtain prebiotics through three different techniques.
Separation from natural resources
Traditional dietary sources of prebiotics include: soybeans, bananas, garlic, barley, onion, Jerusalem artichoke tuber, wheat, asparagus, rye, and chicory root. For many prebiotics that occur naturally in plants or dietary foods, Creative Enzymes can produce prebiotics by separating and extracting them.
Chemical synthesis method
For prebiotics obtained from natural sources in insufficient concentrations for production, Creative Enzymes can provide chemical synthesis method to help our clients obtain the desired compounds. We synthesize some prebiotic components that are not readily available from nature through a number of chemical or biochemical reactions such as hydrolysis, transgalactosylation, isomerization, fructosyl transfer, reduction and oxidation.
Enzymatic reaction method
Many prebiotics are manufactured by enzymatic hydrolysis or transfer reactions using biotechnology. For example, isomalto-oligosaccharides, oligofructose, oligolactose, oligochitosan, etc. are produced by enzymatic methods. Creative Enzymes utilizes various raw materials such as lactose, sucrose, starch and xylan. They are produced by the transgalactosylation activity of the respective carbohydrates. We offer several common enzymatic reaction methods for the production of different types of prebiotics, including but not limited to the following.
| Types of Prebiotics | Process of Production |
|---|---|
| Galacto-oligosaccharides | Trans-galactosylation activity of β-galactosidase using lactose. |
| Lactosucrose | Trans-fructosylation activity of β-fructofuranosidase using a mixture of lactose and sucrose. |
| Lactulose | Trans-galactosylation of lactose by β-galactosidase/β-glucosidase. |
| Fructo-oligosaccharides | Transfructosylation activity of β-fructofuranosidase using sucrose or hydrolysis of inulin by endoinulinase. |
| Isomaltulose (palatinose) | Palatinose synthesis by palatinose synthetase and then intermo lecular dehydration of palatinose. |
| Malto-oligosaccharides | Hydrolysis of starch into maltose by α-amylase and β-amylase, followed by transglucosylation of maltose using α–glucosidase. |
| Isomaltose oligosaccharide | Debranching of starch by isoamylase or pullulanase, followed by hydrolysis of oligosaccharide by α-amylase. |
| Isomaltotriose | Hydrolysis of dextran by dextranase. |
| Xylo-oligosaccharides | Hydrolysis of xylan by xylanase. |
- Identification and Characterization of Prebiotics
Creative Enzymes uses a variety of techniques to accurately characterize isolated prebiotics. Our methods primarily include the determination of the structure using colorimetry, chromatography, optical rotation, and spectroscopic analysis to provide our clients with high-purity prebiotics. In addition, we provide graphical analysis of the characterization results.
Service Process of Creative Enzymes
Creative Enzymes offers one-stop services for prebiotic production and evaluation services, providing our clients with time-saving and cost-effective solutions.
Creative Enzymes is a professional and experienced supplier and service provider of probiotics and related products. We are committed to applying our science to make prebiotics an interesting and useful ingredient in the development of new functional foods. To get more information, please contact us and we will be happy to serve your research demands.
Reference
- Davani-Davari, D.; et al. Prebiotics: definition, types, sources, mechanisms, and clinical applications. Foods. 2019, 8(3): 92.
