Isolation and Purification of Probiotics
The human mouth, intestines, vagina and some fermented foods are inhabited by a large number of bacteria. Certain strains isolated and extracted from the intestines and feces of healthy humans, or from foods or fermented foods with a long history of safe consumption that have beneficial effects on the human body or other microecological systems, can be used as alternative strains of probiotics.
Creative Enzymes offers a variety of probiotic isolation and purification methods to help clients screen for potentially useful probiotic strains.
Isolation and Purification of Strains
The process of obtaining from a mixed microbial community containing only one type or strain of microorganism is called the isolation and purification of probiotics. The research of single strains of probiotics usually requires the isolation of specific microorganisms from a mixed microbiota by isolation and purification techniques, and care must be taken at all times to keep the microorganisms "pure" and prevent the introduction of other microorganisms.
Probiotics Isolation and Purification Methods Provided by Creative Enzymes
Creative Enzymes provide isolation and purification methods that can be used to isolate pure cultures from mixed colonies. We isolate potential probiotic strains from human and animal oral cavity, intestinal contents, feces or other micro-ecological environments primarily by repeated incubation on appropriate, nutrient-rich media or selective media.
We use a variety of methods to separate individual strains so that when they multiply, each strain forms a discrete colony.
| Isolation and Purification Strategies | Methods | |
|---|---|---|
| Isolation and purification by solid media | Dilution pour plate method | Spread plate method |
| Streak plate method | Dilution shaking tube method | |
| Isolation using selective media | Direct separation using selection plates | Enrichment culture method |
| Other methods | Isolation and purification by liquid medium | Single spore isolation method |
| Binary cultures | ||
Probiotic Isolation and Purification Process of Creative Enzymes
Creative Enzymes provides one-stop solutions for strain isolation and purification, including gene sequencing, culture condition screening, strain isolation and purification, molecular identification of pure bacteria and expansion. We are committed to using our expertise to provide our clients with pure strains that have been screened and validated.
Fig. 1 Process of probiotic isolation and extraction.
Creative Enzymes is a professional and experienced probiotic supplier and service provider. We offer a variety of probiotic isolation and purification methods that can provide solutions for our clients to discover valuable potential probiotic strains. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.
