Fermentation of Probiotics

Probiotic fermentation is an important part of the probiotic development process. Creative Enzymes has many years of experience in probiotic fermentation and a full range of fermentation equipment, which allows us to provide professional, customized fermentation services for the development of probiotic products. Our fermentation systems follow high quality industry standards to ensure quality development services for our clients, and can meet any demands of our clients in the pharmaceutical, food, and agricultural industries.

Fig. 1 Main factors affecting the viability of probiotics from production to the gastrointestinal tract. (Lacroix, 2007)

Our Services

• Strain Selection and Evaluation

Starting from our clients' demands, we select production strains with the required functions and benefits for product development through high-throughput screening techniques.

• Fermentation Process Optimization

As probiotics face various stresses from the environment and processing during fermentation, it seriously affects the growth, physiological function and metabolic activity of probiotics, which in turn affects their function. Therefore, during the actual fermentation process, strains are often improved and enhanced to meet the needs of their commercial production. We provide fermentation optimization solutions at multiple scales, including strain modification, media optimization, mass spectrometry, and many other aspects, depending on the client's fermentation end-product needs.

• Large-scale Manufacturing

We make precise calculations according to the actual fermentation demands and scale of our clients, and meet any fermentation scale of our clients with our advanced fermentation equipment to achieve mass production.

Our Methods

Creative Enzymes draws on our extensive expertise in microbial fermentation and biotechnology to optimize strains from both upstream process optimization and downstream process optimization. We are committed to providing high quality probiotic products by continuously optimizing the fermentation process through a variety of optimization strategies and by focusing on product stability and purity as well as yield.

• Upstream Process Optimization

Upstream process optimization focuses on the modification and metabolic enhancement of the strains using various molecular and synthetic biology approaches. Our approaches mainly consist of traditional evolutionary engineering strategies, as well as functional enhancement strategies based on systems biology and reverse metabolic engineering.

Evolutionary Engineering Enhanced Strategies to Improve Probiotics

Biological approaches such as mutagenesis, adaptive evolution and genome modification are mainly used to improve the characteristics of the strains.

Improving Probiotics Based on Systems Biology and Reverse Metabolic Engineering

By using a combination of systems biology and reverse metabolic engineering to rapidly integrate a "phenotype-genotype-phenotype" recombinant strain, ideal phenotypic strains can be obtained.

In addition, we optimize the medium formulation and change the fermentation conditions to improve the purity and productivity of the product.

• Downstream Process Optimization

Downstream process optimization focuses on analyzing the components in the fermentation broth through chromatography, membrane technology, crystallization and distillation, and then providing customized downstream process optimization solutions through the demands of different target products. Our advanced technologies and attention to detail ensure that process optimization is achieved.

Our Facilities

Our laboratories are equipped with a wide range of fermenters of different sizes with capabilities ranging from small to large production fermentations to meet the different demands of our clients. Our built-in, fully automated sensor and monitor system helps maintain optimal growth conditions for the strain, significantly improving fermentation quality and efficiency.

Our Service Process

• Design and evaluation of fermentation programs.
• Selection and optimization of starting strains.
• Development of culture media and fermentation process optimization.
• Pilot scale fermentation, isolation and lyophilization.
• Large scale fermentation and subsequent processing.

Creative Enzymes is a professional and experienced probiotic supplier and service provider. We provide personalized probiotic fermentation services according to the demands of our clients. To get more information, please contact us and we will be happy to serve your research demands.

Reference

1. Lacroix, C.; et al. Fermentation technologies for the production of probiotics with high viability and functionality. Current Opinion in Biotechnology. 2007, 18(2): 176-183.