Identification of Probiotics by Fluorescence Spectroscopy
Fluorescence spectroscopy has been widely and effectively applied in microbial identification and characterization. Creative Enzymes leverages our extensive expertise to provide fluorescence spectroscopy for strain identification using intrinsic fluorescence of probiotic bacteria to support our clients' challenges in probiotic safety assessment.
Introduction of Fluorescence Spectrometry
Probiotics function as a living microorganism. Live bacteria contain a variety of intracellular biomolecules associated with energy responses that have specific excitation and emission wavelength spectra that characterize their specific fluorescence. Thus, creative enzymes use the inherent fluorescent properties of living bacteria as probes for bioassay and characterization. These endogenous fluorescent moieties include protein amino acids, other amino acids, nucleic acids and coenzymes. Fluorescence spectroscopy has shown a powerful ability to distinguish between bacteria belonging to different taxonomic families.
The Process of Identifying Probiotics by Fluorescence Spectroscopy
As a provider of accurate and reliable probiotic identification services, Creative Enzyme is committed to providing our clients with one-stop microbial identification services, including sample handling, screening of test conditions, test execution and data analysis. We meet all your demands with top-notch technology and attentive services.
In the laboratory of Creative Enzymes, we use fluorescence spectroscopy and multivariate analysis for the rapid detection and classification of strains. Our identification process mainly includes the following.
- Samples of each strain cultured under suitable conditions were diluted with saline for analysis.
- Fluorescence spectra in the range of 200-700 nm were collected at 0.5 nm intervals on a fluorescence spectrometer.
- A simultaneous scanning technique is used to find the optimal excitation (λ(ex)) and emission (λ(em)) wavelengths for individual bacteria and to set the appropriate wavelength interval.
- Classification of strains based on simultaneous scanning results.
- For strains with similar emission spectra that were difficult to classify, the application of principal component analysis (PCA) to the fluorescence spectra allowed the bacteria to be successfully classified by genus and their concentrations to be determined.
Advantages of Fluorescence Spectrometry
Fluorescence spectroscopy has proven to be a good tool for probiotic identification. It has shown the following advantages in probiotic identification.
- Fast and high sensitivity.
- Highly selective.
- Minimal sample preparation.
Creative Enzymes is a professional and experienced probiotic supplier and service provider. Creative Enzymes utilizes our technology and expertise to provide our clients with multi-strategy strain identification methods, including fluorescence spectroscopy. We are committed to providing our clients with efficient and professional identification services to facilitate your project. To get more information, please contact us and we will be happy to serve your research demands.
