Genotoxicity Tests of Probiotics
Genotoxicity assessment is an important component of probiotic safety assessment. As a trusted partner for probiotic manufacturers and functional food producers, Creative Enzymes not only provides professional genotoxicity testing required for product safety, but also provides sound advice to help our customers develop safe, high-quality probiotic products.
The Genotoxicity Tests We Provide
Creative Enzymes assesses the genotoxicity of probiotics using a variety of rapid and classical analytical methods that follow international standards. Our scientific team provides our clients with reliable and timely evaluation reports through careful test design using strict quality standards. Our genotoxicity tests including, but is not limited to, the following.
- Bacterial reverse mutation test
The mutagenic potential of probiotics has been assessed by bacterial reverse mutagenesis assay (Ames Test). The Ames test by plate doping is the most commonly used adaptive technique for testing the mutagenic capacity of probiotics. We used various Salmonella typhimurium LT2 and E. coli WP2 strains for bacterial mutation testing. Creative Enzymes uses this test to provide an initial screening of probiotic products for potential adverse effects. Our test provides reproducible results in the shortest possible time and minimizes workload while avoiding problems, errors and duplicate tests.
- In vivo mammalian cell micronucleus test
The micronucleus test is used primarily to identify substances that cause cytogenetic damage that results in the formation of micronuclei containing lagging chromosome segments or whole chromosomes. Creative Enzymes selects the appropriate pathway for the rodents to ingest the probiotic to be tested. We detect damage to the chromosomal or mitotic apparatus of red blood cells caused by the product to be tested by analyzing red blood cells sampled from the bone marrow/peripheral blood cells of animals. We offer our clients a one-stop shop for the entire process of testing, including animal grouping, administration, collection, preparation and staining of bone marrow and/or blood cells.
Fig. 1 In vivo mammalian cell micronucleus testing process of Creative Enzymes.
- Chromosome aberration test
Chromosomal aberration tests are used to assess the fragmentogenicity of chemical and physical reagents, i.e. the ability of these reagents to cause chromosomes to break and produce microscopically visible fragments or structural rearrangements. Scientists at Creative Enzymes performed the test in sexually mature mice. The product to be tested and the control are administered continuously by suitable means. The test animals were sacrificed and sampled on the 14th day after the first dose. The genotoxicity of the probiotic products was evaluated and tested through a series of processes such as sample preparation, staining, and microscopic observation.
Creative Enzymes is a professional and experienced probiotic supplier and service provider. Creative Enzymes uses our science to provide our clients with genotoxicity data to support the identity and viability of their probiotic products. We combine the criteria for each test with the client's demands to provide a customized evaluation service. To get more information, please contact us and we will be happy to serve your research demands.
