Culture of Probiotics

Probiotic cultures often have complex nutritional requirements. Although different probiotics grow differently and require different conditions for growth, there are some common points to follow in the design of the media used for the growth of all probiotics. Most probiotics require carbohydrates, amino acids, peptides, fatty acid esters, salts, nucleic acid derivatives and vitamins for growth.

Creative Enzymes is committed to applying our science to provide customized solutions for the efficient cultivation of probiotics.

Fig. 1 Screening of probiotic culture conditions.

Nutritional Requirements of Probiotics

Carbon Sources

Carbon source refers to the nutrients of the carbon element type required to satisfy the growth and reproduction of microorganisms. Simple inorganic carbon-containing compounds, more complex organic matter, complex organic macromolecules, and natural carbon-containing substances (beef paste, peptone, peanut cake powder, molasses, petroleum, etc.) are all sources of carbon that can be used by different microorganisms.

Nitrogen Sources

Nitrogen sources are nutrients that provide nitrogen for microbial growth and reproduction. For example, Lactobacillus proteolytic ability and amino acid synthesis ability is weak, so in artificial culture, it is necessary to add organic nitrogen sources containing a variety of peptide amino acids, such as beef paste, yeast paste, peptone or tomato juice.

Energy Sources

Any substance that provides the energy required for the life activity of microorganisms is called energy. Energy sources vary depending on the microbial species. For some microorganisms, the carbon sources can also be used as an energy sources.

Growth Factors

Growth factors are a class of trace organic substances necessary for the regulation of normal microbial metabolism. When using the medium for probiotic culture, it is necessary to add suitable growth factors to make the probiotic strain grow well and get a strong strain.

Inorganic Salts

Inorganic salts are indispensable for the life activities of probiotic bacteria. The main functions of inorganic salts are to constitute the components of the bacterium, to act as components of enzymes, enzyme activators or inhibitors, to regulate the osmotic pressure of the medium, to regulate the pH value and redox potential, etc.

Water

Water is the solvent for cells to absorb nutrients and excrete metabolites and the medium for biochemical reactions. A certain amount of water is also necessary to maintain cellular osmotic pressure and regulate intracellular temperature.

Fig. 2 Nutrient screening for the growth of probiotics.

Creative enzymes screen a series of factors required for the nutritional growth of different probiotic bacteria and compare them to screen for suitable growth formulas that promote good growth of probiotic strains.

Factors Affecting the Growth of Probiotics

• Oxygen
• Temperature
• pH
• Osmotic pressure

The Efficient Culture Solutions Creative Enzymes Provide for Probiotics

• In-depth understanding of the nutritional requirements and metabolic properties of different probiotics.
• Find and design customized media formulations that are rich in nutrients, comprehensive in elements, easy to obtain ingredients and effective in results.
• The physiological conditions for optimal growth and metabolite production are created and tailored according to the metabolic and growth characteristics of different probiotics.

Creative Enzymes is a professional and experienced probiotic supplier and service provider. We are committed to providing customized solutions for quality growth formulations of different probiotics. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.