Biochemical-Based Identification System
Creative Enzymes offers automated identification systems based on biochemical methods mainly consisting of the API system and the Vitek system. These systems serve as the "gold standard" for microbial identification methods, providing an efficient and sensitive automated means of detection. Our dedicated technical team is committed to providing scientific and reliable insights into our clients' probiotic product evaluation and development through our advanced testing platform.
API System
The API system is a microvolume biochemical identification system that can be thought of as a grouped library of small biochemical tests. The system provides accurate identification results based on an extensive database. One of the most representative ones is API 20E.
Fig. 1 Identification of Staphylococcus aureus using the API system. (Gabisonia, 2019)
- Working Principle
The API system performs digital taxonomic identification based on the physiological and biochemical identification results of microorganisms. The authentication system is a long card with 20 compartments arranged on it. According to the physiological and biochemical characteristics of microorganisms, each compartment is respectively added with a trace amount of configured medium, reaction substrate, reagent and other substances.
The different tests in API 20E mainly include:
| ONPG(o-nitrophenyl-b-D-galactopyranoside) test | ADH (arginine dihydrolase) | LDC (lysine decarboxylase) test | ODC (ornithine decarboxylase) test |
|---|---|---|---|
| CIT (citrate) utilization test | H2S (hydrogen sulfide) test | URE (urea) hydrolyzation test | TDA (Tryptophan deaminase) |
| IND (Indole)test | VP (Voges-Proskauer) test | GEL (gelatin) hydrolysis test | GLU (glucose) fermentation test |
| MAN (mannitol) fermentation | INO (inositol) fermentation test | SOR (sorbitol) fermentation test | RHA (rhamnose) fermentation test |
| SAC (sucrose) fermentation test | MEL (melibiose) fermentation test | AMY (amygdalin) fermentation test | ARA (Arabinose) fermentation test |
- API System Test Process
During the test, the bacterial solution of the microorganism to be tested was added to the and incubated for 24-48 h, and the various reactions on the identification card were observed. Combined with other complementary indicators such as morphology, the results were then coded and analyzed to finally obtain the identification of the strain.
Vitek Systems
The Vitek system is an advanced and highly automated system whose performance, accuracy and reliability have been extensively evaluated and proven. The Vitek system is available in 3 main versions, Vitek 2, Vitek 2 Compact and Vitek MS. These systems identify microbes based on the color they produce using the electricity generated by the substrate and microbial proteins in the card.
Fig. 2 The Vitek systems.
- Working Principle
Take the Vitek 2 system, for example, which is available in three formats (Vitek 2 Compact, Vision Technology 2 and Vitek 2 XL). Although they differ in terms of increased capacity and level of automation, all three systems are suitable for the same colorimetric reagent cards.
Different types of Vitek test cards contain a variety of biochemical reaction wells. When the inoculum is injected, the system performs all steps automatically. The optical system of Vitek 2 combines a multi-channel fluorometer and photometric readout to record fluorescence, turbidity and colorimetric signals. The system allows kinetic analysis to be performed by reading each test every 15 min to provide accurate and efficient test reports.
- Vitek 2 System Test Process
- Prepare bacterial suspensions by picking pure cultures from the plates to make bacterial suspensions by turbidimetry with photoelectric turbidimeter, and prepare to a comparable turbidity.
- Bacterial liquid inoculation and closure by system filling machine.
- The incubation is performed automatically by the thermostat and the readout is automatic.
- Automatic report printing after the test is completed.
Creative Enzymes is a professional and experienced supplier and service provider of probiotics. We provide our clients with a wide range of scientifically proven, advanced technology methods to help them develop better products. To get more information, please contact us or fill out the online inquiry below, we will be happy to serve your research demands.
Reference
- Gabisonia, T.; et al. Fersis-Phage against Purulent-Inflammatory (Staphylococcal, Streptococcal) Pathologies. Archives of Clinical Microbiology. 2019, 10(1): 0-0.
