Detection of Intestinal Flora
The intestinal flora provides nutrients to the body, regulates metabolism, regulates intestinal epithelial development and induces innate immunity, and its function is equivalent to an important "organ" of the human body. Therefore, the detection and research of intestinal flora is important for solving human diseases and other health problems. Creative Enzymes uses our technologies to conduct real-time, comprehensive and systematic assessment of the subject's intestinal flora, which can help clients understand their health status scientifically and customize nutritional health solutions more precisely.
What We Test?
Creative Enzymes' intestinal flora testing service provides comprehensive information on the overall condition of the gut flora, mainly in the following areas.
- Detection and analysis of the species and abundance of intestinal flora: probiotics, conditionally pathogenic bacteria, and pathogenic bacteria.
- Pathogenic bacteria in the gut and disease risk assessment.
- Assessment of the main nutrients: proteins, carbohydrates, lipids and vitamins.
- Evaluation of short-chain fatty acids: acetic acid, propionic acid, butyric acid.
Application of Intestinal Flora Detection
Intestinal flora has received increasing attention due to its relationship with disease and human health. Creative Enzymes is committed to applying our expertise to help our clients make progress in the following researches.
- Look for differences in intestinal flora between the disease and healthy groups.
- Research the effects of pharmacological or dietary interventions on intestinal flora.
- Research the changes in intestinal flora in the same individual at different developmental stages or in different disease development processes.
- Research of the flora of different body parts of the same individual.
Our Detection Technologies
Creative Enzymes offers proven intestinal flora detection solutions through a variety of molecular biology techniques, mainly including the following methods.
- 16S rDNA amplicon sequencing
Creative Enzymes detects 16srDNA of intestinal microorganisms by high-throughput sequencing technology. The resulting large amount of data is then analyzed by bioinformatics tools to reveal the types of microbes in the intestinal and their relative abundance and evolutionary relationships. We are committed to providing our clients with comprehensive testing solutions, including the entire process of sample DNA extraction, PCR amplification, library construction, high-throughput sequencing, and bioinformatics analysis.
- Fluorescent quantitative PCR
The fluorescence quantitative PCR technique allows for the quantification of a particular class of bacteria in the sample, helping clients to research the diversity of intestinal microorganisms and the relationship between intestinal microbes and the environment. Our one-stop testing process mainly includes DNA extraction, specific primer determination, standard plasmid construction, standard curve construction, fluorescence quantitation detection and calculation.
Our Testing Process
Creative Enzymes provides one-stop services for enteric flora testing. Our experienced scientists first isolate and culture the microorganisms in the samples. Then, through our advanced detection and analysis platform, we provide our clients with relevant information on the species and abundance of intestinal microorganisms, and investigate the function and role of intestinal microorganisms at the strain level.
Creative Enzymes is a professional and experienced probiotic supplier and service provider. We are committed to using our comprehensive testing and analysis platform to help our clients scientifically identify the composition of individual gut flora and potential disease risk. To get more information, please contact us and we will be happy to serve your research demands.
