Gene Mutation Service
Mutagenesis is an effective strategy to improve and enhance the performance of probiotics, making them more suitable for industrial use. With our experience in probiotic development, Creative Enzymes helps our clients optimize their strains and accelerate their high-end product development projects through multiple mutagenesis techniques and compound mutagenesis techniques that combine different mutagenesis methods.
Introduction of Mutagenesis Technology
Mutagenesis induces mutations in the genes of strains by artificial measures, mainly chemical mutagenesis, physical mutagenesis and site-directed mutagenesis. Induced mutations produced by physical or chemical mutagens are changes in genes after contact with mutagens and environmental causes that produce non-site-specific random mutations. Conventional mutagenesis methods are to some extent easy to perform and can increase the rate of resistance mutations. For example, Lactobacillus casei LC2W was used as the starting strain for nitrosoguanidine and UV mutagenesis, and the mutagenic strains LC2W-NTG-12 and LC2W-UV-11 were screened to show high acid production and acid tolerance performance. Site-directed mutagenesis is a technique for creating a library of genetic information by introducing a mutation into a DNA sequence. This technique promotes improved strain performance by generating specific DNA mutations (including point mutations, deletion mutations and insertion mutations).
Our Methods
Creative Enzymes offers clients a variety of mutagenesis methods that can alter intracellular gene sequences to optimize genetic traits and obtain desired high yielding, high quality strains. Most of these methods have higher mutation rates and may result in multiple complex types of DNA damage and mutations.
- Chemical mutagenesis
Chemical mutagenesis mainly uses some chemical mutagens. Most chemical mutagens are base analogs, base modifiers and shift mutagens, including bromodeoxyuridine (BrdU), alkylating agents, agents that form DNA adducts, DNA intercalating agents, DNA crosslinkers, etc.
- Physical mutagenesis
Physical mutagenesis methods mainly utilize UV, α-rays, β-rays and γ-rays. In addition, we also offer new physical mutagenesis methods based on atmospheric room temperature plasma (ARTP).
- Site-directed mutagenesis
Site-directed mutagenesis is a method of mutating the DNA sequence of a strain. In the Creative Enzymes' lab, our scientists use molecular biology methods to synthesize short DNA primers that contain the desired mutation site and are complementary to the template DNA surrounding the mutation site. The single-stranded primers are then extended using DNA polymerase, and the replicated gene contains the mutation site. Mutations can be single base changes, multiple base changes, deletions or insertions.
Workflow of Creative Enzymes
Fig. 1 Project workflow of Creative Enzymes.
Creative Enzymes is a professional and experienced probiotic supplier and service provider. Our mutagenesis services provide a rapid, cost-effective way to introduce genetic mutations into strains to improve their performance and activity and better assist our customers in their probiotic product development. To get more information, please contact us and we will be happy to serve your research demands.
