In Vivo Safety Assessment of Probiotics

The in vivo safety of probiotics requires animal models to predict their potential side effects after oral administration. Creative Enzymes' scientific team has developed a complete probiotic in vivo safety assessment program through our experience and expertise to support the safety information required for any probiotic.

Why Should I Conduct the In Vivo Safety Assessment of Probiotics

In vitro safety testing is primarily concerned with the identification of false negative strains. However, virulence signature may not be active under specific assay conditions, resulting in non-detection. Since virulence is a complex phenomenon, most of the time it requires an active interaction with the host to be triggered. From a scientific and ethical point of view, in vivo animal models are the most appropriate. Typically, researches using animal models should be conducted with in vitro tests fully completed.

Strategies for In Vivo Safety Assessment of Probiotics at Creative Enzymes

Creative Enzymes, a well-known provider of probiotic safety assessment services, has developed a comprehensive assessment program to respond to all the different concerns regarding probiotic safety. The in vivo tests we provide mainly include the following.

Toxicological Assessment of Probiotics

Toxicological assessment of creative enzymes uses a variety of toxicological tests to evaluate the potential harm of probiotics to the host. Our tests focus on the genotoxicity and oral toxicity of probiotics to help our clients better understand the safety of the strains.

Assessment of Probiotic Translocation Potential

In vivo tests of probiotics mainly focus on the potential for bacterial translocation. For most enteric pathogens, transit across the epithelium is considered to be a prerequisite for pathogenicity. Creative Enzymes uses our science, combined with animal models, to research the potential translocation ability of probiotics and the resulting side effects to help our clients obtain comprehensive safety information on probiotics.

Assessing the Effect of Probiotics on Seriously Ill or Immunocompromised Hosts

In terms of safety researches of probiotics on immunocompromised models, creative enzymes uses a variety of models including juvenile, adult, innate immune deficiency and acquired induction to provide a comprehensive and integrated analysis of the potential adverse effects of probiotics on immunodeficient hosts.

Animal Models Provided by Creative Enzymes for Assessing the Safety of Probiotics

Different animal models contribute to the understanding of probiotic functions and play an important role, especially in the research of probiotic mechanisms of action, health effects and safety evaluation. To confirm the in vivo effects of probiotics, Creative Enzymes provides our clients with a variety of appropriate and validated animal models prior to human trials.

We mainly use in vivo models of vertebrates, most commonly mice and rats. Other domestic animals or fish have been used in in vivo tests of probiotics for animals. In addition, disease animal models are also used in our laboratory to detect any potential pathogenicity of the candidate probiotic strains.

Creative Enzymes is a professional and experienced probiotic supplier and service provider. We are committed to helping our clients select appropriate animal models and provide customized research strategies to provide comprehensive in vivo safety assessment services. To get more information, please contact us and we will be happy to serve your research demands.