Hemolysis Test of Probiotics

Hemolysis is one of the pathogenic mechanisms of pathogenic bacteria, so the hemolytic effect of probiotics needs to be tested to ensure their safety. Creative Enzymes utilizes our extensive test platform to provide test analysis covering all probiotic safety regulatory requirements for our clients' probiotic safety evaluations, including hemolysis test.

Introduction of Microbial Hemolysis

Hemolysis is the rupture of red blood cells accompanied by the release of hemoglobin, which can have adverse health effects on the individual. Some bacteria produce hemolysins during growth, which cause red blood cells to rupture and lyse. When bacteria that cause hemolysis grow on blood plates, a hemolytic ring is created around these bacteria. Different bacteria produce different hemolysins and have different hemolytic capacities, thus showing different hemolysis on the blood plate.

Fig. 1 Different types of bacterial hemolysis on blood agar.

Common Hemolysis Phenomena Include:

Why Should I Conduct Hemolysis Test for Probiotics

In general, some pathogenic bacteria such as Escherichia coli, Bacillus cereus, etc. have been shown to produce hemolysins associated with pathogenesis. Currently, there is no research evidence to suggest that Lactobacilli and Bifidobacteria have hemolytic capacity. However, it has been shown that some Lactic acid bacteria can express alpha-hemolysin, making it unsafe for human consumption.

Therefore, it is necessary to incorporate hemolysis testing of bacteria into account in the safety evaluation of probiotics. Creative Enzymes is a good partner to provide hemolysis testing services for your probiotics products, and we are committed to providing you with scientific and reliable test results.

Hemolysis Test Procedure in Creative Enzymes

Creative Enzymes provides fast and efficient hemolysis test to help our clients determine whether their probiotic products meet safety regulatory requirements. What you need to do is to provide us with the samples to be tested and we can provide you with accurate test results according to the following process.

• The probiotic culture to be tested was scribed onto blood agar medium containing 5-10% sheep blood.
• The strains were incubated under suitable conditions (incubation at 37°C for 24 hours).
• The isolates were examined for the presence of clear areas around the isolates. Clear areas are considered β-hemolysis, green areas are considered α-hemolysis, and areas not showing hemolysis are γ-hemolysis. Strains showing β or α hemolysis were excluded and those showing γ hemolysis were probiotics without hemolytic activity.

Creative Enzymes is a professional and experienced probiotic supplier and service provider. We help our clients save time and effort by providing them with high-quality testing and analysis services throughout the entire process according to their demands. To get more information, please contact us and we will be happy to serve your research demands.